anti myo7a proteus  (Novus Biologicals)

Journal: bioRxiv

Article Title: Tbx2 is essential for cochlear inner hair cell development and regeneration

doi: 10.1101/2022.05.05.490782

Figure Lengend Snippet: (A-A’’’) Triple staining of Myo7a, Tdtomato and Sox2 in control cochleae of Plp1-Ai9 mice that are administered with TMX (red arrows) and TMP (blue arrows), and analyzed at P7. Orange arrows mark one IBC/IPh that is Tdtomato+/Sox2+/Myo7a-. (B) The simple cartoon illustrating the genetic model to conditionally induce transient Atoh1 and permanent Tbx2. Please also refer to for details. (C-F’’’) Triple staining of HA (Atoh1), Tdtomato and V5 (Tbx2) in Plp1-TAT mice that are subjected to different treatment procedures and analyzed at different ages, as illustrated on the right sides. Orange arrows in (C-C’’’) label one IBC/IPh that expresses high protein levels of Tdtomato and Tbx2, but weak Atoh1. Differently, orange arrows in (D-D’’’) point to one IBC/IPh expressing high protein levels of Tdtomato, Tbx2 as well as Atoh1 because Atoh1 is stabilized with additional TMP treatment. Atoh1 is reversed to a weak level in Tdtomato+/Tbx2+ cells (orange arrows in E-E’’’) if mice are analyzed at P7 (3 days after TMP), but can be recovered to a high level in Tdtomato+/Tbx2+ cells (orange arrows in F-F’’’) if the third TMP is given 3 hours before final analyzing at P7. (G-G’’’) Triple staining of Myo7a, Tdtomato and Sox2 in cochleae of Plp1-TAT mice at P42. Arrows point to the Tdtomato+/Sox2+ cell that does not express Myo7a and is defined as IBC/IPh failing to become HCs. Scale bar: 20 μm (A’’’, F’’’ and G’’’).

Article Snippet: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse, 1:500, MCA1360, Bio-rad), anti-Prestin (goat, 1:1000, sc-22692, Santa Cruz), anti-vGlut3 (rabbit, 1:500, 135203, Synaptic Systems), anti-Otoferlin (mouse, 1:500, ab53233, Abcam), anti-Myo7a (rabbit, 1:500, 25-6790, Proteus Bioscience), anti-Ctbp2 (mouse, 1:200, 612044, BD Biosciences), anti-Sox2 (goat, 1:1000, sc-17320, Santa Cruz), anti-Myo6 (rabbit, 1:500, 25-6791, proteus-biosciences), anti-Slc7a14 (rabbit, 1:500, HPA045929, Sigma-Aldrich).

Techniques: Staining, Expressing

Journal: bioRxiv

Article Title: Tbx2 is essential for cochlear inner hair cell development and regeneration

doi: 10.1101/2022.05.05.490782

Figure Lengend Snippet: (A-B’’’) Triple staining of vGlut3, Tdtomato and Otoferlin in cochleae of control Plp1-Ai9 (A-A’’’) and Plp1-TAT (B-B’’’) mice at P42, both of which are treated TMX at P0 and P1 and TMP at P3 and P4. Arrows in (A-A’’’) and (B-B’’’) mark one IBC/IPh that is Tdtomato+/vGlut3-/Otoferlin-, and one new IHC that is Tdtomato+/vGlut3+/Otoferlin+, respectively. (C-D’’) Double staining of Myo7a and Tdtomato in cochleae of Plp1-Ai9 (C-C’’) and Plp1-TAT (D-D’’) mice. Likewise, arrows in (C-C’’’) and (D-D’’) mark one IBC/IPh that is Tdtomato+/Myo7a-, and one new IHC that is Tdtomato+/Myo7a+, respectively. (E-F) Quantification of new IHC numbers (E) or percentage (F) of new IHCs among all Tdtomato+ cells, using either Myo7a or vGlut3 as a marker at P42. Scale bar: 20 μm (B’’’ and D’’).

Article Snippet: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse, 1:500, MCA1360, Bio-rad), anti-Prestin (goat, 1:1000, sc-22692, Santa Cruz), anti-vGlut3 (rabbit, 1:500, 135203, Synaptic Systems), anti-Otoferlin (mouse, 1:500, ab53233, Abcam), anti-Myo7a (rabbit, 1:500, 25-6790, Proteus Bioscience), anti-Ctbp2 (mouse, 1:200, 612044, BD Biosciences), anti-Sox2 (goat, 1:1000, sc-17320, Santa Cruz), anti-Myo6 (rabbit, 1:500, 25-6791, proteus-biosciences), anti-Slc7a14 (rabbit, 1:500, HPA045929, Sigma-Aldrich).

Techniques: Staining, Double Staining, Marker